Germ-Free Mouse Facility

Researcher in germ-free mice facility using microscopeEstablished in 2004, the germ-free mouse facility, part of the Unit for Laboratory Animal Medicine, provides services to the entire University of Michigan community and can also ship germ-free mice to locations worldwide. 

Primary and collaborative research projects include studies investigating inflammatory bowel disease and cancer, host microbiome interactions, infectious disease pathogenesis, and mammalian and bacterial metabolism, among others. The staff also performs research and training in gnotobiotic technology and methods.

A complete list of recent publications that include U-M’s Germ-Free Mouse Facility can be found on PubMed.

Users must have an approved animal use protocol from the Institutional Animal Care & Use Committee prior to utilizing Germ-Free Mouse Facility services and facilities.

germ-free mouse

Mice can be used in our facility or shipped to other facilities in germ-free shipping containers.

Available strains include:

  • C57BL/6
  • BALB/c
  • Swiss-Webster (outbred)
  • IL-10 knockout (B6.129P2-Il10tm1Cgn/J)
  • RAG-I knockout (B6.129S7-Rag1tm1Mom/J)
  • Others may be available upon request

Several varieties of germ-free and barrier housing are available:

Sterile:

  • Trexler-type soft-sided isolators can be used for breeding colonies and long-term germ-free or gnotobiotic housing

Barrier:

  • Microisolator cages can be kept sterile in biosafety cabinets for short-term experiments
  • Individual Isocages ® (Techniplast) can maintain germ-free or gnotobiotic conditions for long-term experiments

Germ-Free Mouse Facility housing options

  • Inoculation (oral and parenteral)U-M Germ-Free Mouse Facility animal husbandry
  • Sample collections, including:
    • Blood
    • Tail
    • Feces
    • Urine
    • Body weights
    • Necropsy and tissue collection
  • Technical consultation
  • Other services may be available on request
  • U-M Germ-Free Mouse Facility rederivationRe-derivation of new germ-free strains
  • Consultation:
    • Experimental design
    • Pathology services
    • Histologic interpretation

Contract services may also be available.

Please contact Germ-Free Mouse Facility Director at kateaton@umich.edu with specific inquiries.

Facilities and associated services are offered on a fee-for-service basis. Users are charged for all supplies, technical assistance, and equipment used. Estimates are available upon request.

Germ-Free Animal Procurement Rates

DESCRIPTION RATE EFFECTIVE
C57Bl/6 Germ-Free Mice (per animal) $  65.64 06/30/2015
Swiss-Webster Germ-Free Mice (per animal) $  52.51 06/30/2015
IL-10KO Germ-Free Mice (per animal) $  65.64 06/30/2015
Rag1KO Germ-Free Mice (per animal) $  65.64 06/30/2015

Germ-Free Service Rates

DESCRIPTION RATE EFFECTIVE
5-pack fecal kit $  15.58 06/30/2015
10-pack fecal kit $  16.97 06/30/2015
5-pack serum submandibular collection kit $  18.35 06/30/2015
10-pack serum submandibular collection kit $  21.67 06/30/2015
5-pack whole blood submandibular collection kit $  18.19 06/30/2015
10-pack whole blood submandibular collection kit $  21.36 06/30/2015
Injection kit $  15.15 06/30/2015
Germ-Free per diem (per care day) $  5.59 06/30/2015
Germ-Free shipper $  704.55 06/30/2015
Gas sterilization - small $  15.80 06/30/2015
Gas sterilization - medium $  16.75 06/30/2015
Gas sterilization - large $  22.56 06/30/2015
Sterile mouse box transport kit $  15.85 06/30/2015
Oral gavage w/ feeding needle kit $  20.65 06/30/2015
Port entry/exit $  30.67 06/30/2015

 

* Rates subject to change.

Questions?

Contact ulam-germfree@umich.edu or (734) 615-9420 for additional information, or to learn more about available services.